What is the testing method for amino acids? How to Test Your Patients' Amino Acid Levels?

Every living being uses Amino acids to make proteins. They help grow and repair body tissue and boost your immune system. It is crucial to balance Essential Amino acid levels throughout the day. There are six tests for determining the functional groups in amino acids. They are:

(1) Ninhydrin Test

The free amino group and Carboxylic acid group react with ninhydrin to produce a colored residue.

(2) Biuret Test

Also known as Piotrowski’s Test, the dark violet-colored residue signifies the presence of protein in the solution.

(3) Xanthoproteic Test

This test identifies the presence of an activated benzene ring in the amino acid. Nitric acid is added to 1 ml of amino acid solution, followed by boiling to detect the Essential Amino acid levels.

(4) Millon’s Test

This test is suitable for amino acids containing a phenol group. Millon’s reagent is used in the test to identify protein traces in the sample.

(5) Hopkins-Cole Test

This test is done particularly for tryptophan, the only amino acid containing an indole group.

(6) Nitroprusside Test

The sulfhydryl group reacts with nitroprusside in an alkaline solution to give a red compound.

The labs for Vitamin analysis in Chennai carry out the following tests to analyze the amino acid balance in the body.

A plasma amino acid test is carried out to measure what is being transported during sampling. The specimen is collected after an overnight fasting and then compared with the ref range.

Amino acid panels give a measure of nutritional deficiencies in patients.

Urine amino acids or FMV help detect health issues like dietary protein sufficiency, amino acid balance, gastrointestinal problems, vitamin and mineral deficiencies, kidney and liver dysfunction etc.

Being one of the unsurpassed Amino acid Testing Labs in Chennai, Tamil Nadu Test House follows standard test methods to determine the presence of amino acids and its composition and quantity in food samples. Contact us now.

Comments

Cosmetics Testing labs in Chennai

Tamilnadu Test House Pvt Ltd is an NABL-accredited laboratory as well as an accredited laboratory. Tamilnadu Test House is a well-known lab in the fields of Chemistry, Microbiology, and Biotechnology. We offer services in LCMS, GCMS, HPLC, GC, FT-IR, AAS, TGA, DSC, UV-Vis, and in all spectroscopies, and chromatography instruments. We are offering the following services in Cosmetic 1. Test the cosmetic Products as per BIS standards and certify the Products 2. Chemical analysis of Cosmetic Products 3. Microbial Analysis and Contamination of the Products 4. Heavy metal contamination 5. Organic certification of the Products 6. Allergen testing as per IFRA Standards 7. Comparative study of your product with international brands 8. Shelf life study 9. Preservative efficacy study Please call us at +91 75500530...

What Is Your Skin Type? 5 Steps To Identify What Your Skin Needs To Flourish

Understanding your skin type is essential for establishing an effective skincare routine that addresses your specific needs and concerns. With so many products available in the market, tailoring your approach to your skin type can help you achieve a healthier and more radiant complexion. Normal skin: It typically has a balanced level of oil production, appearing neither excessively oily nor overly dry. Pores are generally small and the skin has a smooth texture. If your skin feels comfortable throughout the day and you rarely experience breakouts or dry patches, you likely have normal skin. A basic skincare routine involving gentle cleansing, moisturizing, and sun protection is usually sufficient for normal skin. Occasional exfoliation and targeted treatments for specific concerns can be added. Oily skin: It tends to produce an excess of sebum, leading to a shiny appearance, enlarged pores, and a higher likelihood of acne and blackheads. If your face becomes oily within a few hours of ...

How do you Analyse vitamin content in food?

Analyzing the vitamin content in food involves several laboratory techniques. Here's an overview of the common methods used High-Performance Liquid Chromatography (HPLC): HPLC is a widely used technique for analyzing vitamins, particularly water-soluble vitamins like vitamin C (ascorbic acid), B vitamins (thiamine, riboflavin, niacin, etc.), and fat-soluble vitamins like vitamins A, D, E, and K. This method separates and quantifies individual vitamins based on their chemical properties. Liquid Chromatography-Mass Spectrometry (LC-MS): LC-MS combines chromatography with mass spectrometry detection to analyze and quantify vitamins in food samples. It offers high sensitivity and selectivity and can be used for both water-soluble and fat-soluble vitamins. UV-Visible Spectrophotometry: UV-visible spectrophotometry is commonly used for analyzing vitamin concentrations, particularly for vitamin A (retinol) and vitamin E (tocopherol), which absorb UV or visible light at specific wav...

Tamilnadutesthouse

Search This Blog