What is the testing method for amino acids? How to Test Your Patients' Amino Acid Levels?

Every living being uses Amino acids to make proteins. They help grow and repair body tissue and boost your immune system. It is crucial to balance Essential Amino acid levels throughout the day. There are six tests for determining the functional groups in amino acids. They are:

(1) Ninhydrin Test

The free amino group and Carboxylic acid group react with ninhydrin to produce a colored residue.

(2) Biuret Test

Also known as Piotrowski’s Test, the dark violet-colored residue signifies the presence of protein in the solution.

(3) Xanthoproteic Test

This test identifies the presence of an activated benzene ring in the amino acid. Nitric acid is added to 1 ml of amino acid solution, followed by boiling to detect the Essential Amino acid levels.

(4) Millon’s Test

This test is suitable for amino acids containing a phenol group. Millon’s reagent is used in the test to identify protein traces in the sample.

(5) Hopkins-Cole Test

This test is done particularly for tryptophan, the only amino acid containing an indole group.

(6) Nitroprusside Test

The sulfhydryl group reacts with nitroprusside in an alkaline solution to give a red compound.

The labs for Vitamin analysis in Chennai carry out the following tests to analyze the amino acid balance in the body.

A plasma amino acid test is carried out to measure what is being transported during sampling. The specimen is collected after an overnight fasting and then compared with the ref range.

Amino acid panels give a measure of nutritional deficiencies in patients.

Urine amino acids or FMV help detect health issues like dietary protein sufficiency, amino acid balance, gastrointestinal problems, vitamin and mineral deficiencies, kidney and liver dysfunction etc.

Being one of the unsurpassed Amino acid Testing Labs in Chennai, Tamil Nadu Test House follows standard test methods to determine the presence of amino acids and its composition and quantity in food samples. Contact us now.

Comments

What is Animal Feed testing ?

Animal feed testing labs in Chennai analyze samples of animal feed to assess their nutritional composition, quality, and safety. Here's an overview of the process involved in animal feed testing: Sample Collection: The first step is to collect representative samples of the animal feed to be tested. Samples should be collected following proper sampling procedures to ensure accuracy and reliability. Preparation of Samples: Once collected, the samples may need to be prepared or homogenized to ensure uniformity and consistency. This may involve grinding, mixing, or sub-sampling the feed samples as needed. Selection of Testing Parameters: Depending on the specific requirements and objectives of the testing, various parameters may be analyzed, including: Nutritional Composition: This includes testing for moisture content, protein content, fat content, fiber content, carbohydrate content, and mineral content. Contaminants : Testing may be conducted to detect contaminants such as afla...

The Attenuated Total Reflectance (ATR) technique is used in FT-IR spectroscopy

Attenuated Total Reflectance (ATR) is a powerful measurement technique used in FT-IR spectroscopy. Let’s explore its applications and how it works: 1. Identification of Chemical Compounds: ATR-FTIR spectroscopy helps identify chemical compounds by analyzing their unique infrared absorption patterns. It’s particularly useful for characterizing organic and inorganic materials. 2. Studying Molecular Structures: ATR provides insights into molecular structures, including functional groups and bonding arrangements. Researchers use it to investigate polymers, proteins, and other complex molecules. 3. Surface Properties Examination: ATR allows surface analysis without extensive sample preparation. It’s valuable for studying thin films, coatings, and interfaces. 4. Analyzing Polymers: Polymer characterization benefits from ATR due to its ability to analyze solid samples directly. Researchers study polymer composition, additives, and degradation. 5. Biomolecule...

Agriculture Testing Labs in Chennai

Tamilnadu Test House Pvt. Ltd. (TNTH) is a leading testing laboratory in India, providing laboratory services in the fields of Pharmaceutical testing, food testing, agriculture testing, environmental testing, etc. We use the latest methods and technology, to identify risk factors, improve quality, and efficiency, and add value to the market. As your testing partner, you can be sure of fast turnaround times and accurate test results demonstrating compliance with statutory requirements Agriculture analysis: Soil Test for Micro Macro content Compost analysis as per FCO (Fertilizer Control order) Bio Fertilizer analysis Nitrogen Fixing Biofertilizers Phosphate Biofertilizers Phosphorus Solubilizing Biofertilizers Phosphorus Mobilizing Biofertilizers Biofertilizers for Micro-nutrients Plant Growth Promoting Rhizobacteria Azadirachtin Content analysis FULVIC ACID/ HUMIC ACID analysis Seaweed (Physical, Chemical, Microbial Analysis) ...

Tamilnadutesthouse

Search This Blog