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What is the testing method for amino acids? How to Test Your Patients' Amino Acid Levels?

Every living being uses Amino acids to make proteins. They help grow and repair body tissue and boost your immune system. It is crucial to balance Essential Amino acid levels throughout the day. There are six tests for determining the functional groups in amino acids. They are:


(1) Ninhydrin Test

The free amino group and Carboxylic acid group react with ninhydrin to produce a colored residue. 

 (2) Biuret Test 

Also known as Piotrowski’s Test, the dark violet-colored residue signifies the presence of protein in the solution. 

(3) Xanthoproteic Test 

This test identifies the presence of an activated benzene ring in the amino acid. Nitric acid is added to 1 ml of amino acid solution, followed by boiling to detect the Essential Amino acid levels.

(4) Millon’s Test 

This test is suitable for amino acids containing a phenol group. Millon’s reagent is used in the test to identify protein traces in the sample.

(5) Hopkins-Cole Test 

This test is done particularly for tryptophan, the only amino acid containing an indole group. 

 (6) Nitroprusside Test

The sulfhydryl group reacts with nitroprusside in an alkaline solution to give a red compound. 


The labs for Vitamin analysis in Chennai carry out the following tests to analyze the amino acid balance in the body.

A plasma amino acid test is carried out to measure what is being transported during sampling. The specimen is collected after an overnight fasting and then compared with the ref range.

Amino acid panels give a measure of nutritional deficiencies in patients. 

Urine amino acids or FMV help detect health issues like dietary protein sufficiency, amino acid balance, gastrointestinal problems, vitamin and mineral deficiencies, kidney and liver dysfunction etc.

Being one of the unsurpassed Amino acid Testing Labs in Chennai, Tamil Nadu Test House follows standard test methods to determine the presence of amino acids and its composition and quantity in food samples. Contact us now.


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