๐ฌ High-Performance Liquid Chromatography (HPLC) is a powerful technique used to separate and quantify synthetic dyes in food samples. ๐งช Brief Procedure: ๐ง Sample Prep: Food sample is homogenized and extracted using water or a suitable solvent. ๐ง Filtration: The extract is filtered to remove particulates. ๐ Injection: A small volume is injected into the HPLC system. ⚗️ Separation: The dyes are separated on a C18 column using a gradient mobile phase. ๐ Detection: Dyes are detected using UV-Vis at specific wavelengths. ✅ Used in quality control to ensure dye levels comply with food safety standards.