🔬 High-Performance Liquid Chromatography (HPLC) is a powerful technique used to separate and quantify synthetic dyes in food samples. 🧪 Brief Procedure: 💧 Sample Prep: Food sample is homogenized and extracted using water or a suitable solvent. 🧂 Filtration: The extract is filtered to remove particulates. 💉 Injection: A small volume is injected into the HPLC system. ⚗️ Separation: The dyes are separated on a C18 column using a gradient mobile phase. 📊 Detection: Dyes are detected using UV-Vis at specific wavelengths. ✅ Used in quality control to ensure dye levels comply with food safety standards.