Tamilnadu Test House offers HPTLC Testing services in the below areas for identification and Quantification of the components. HPTLC is a sophisticated instrument that is used for comparing several samples in parallel, checking for adulteration, Purity analysis, and quantifying marker compounds.
Botanical industry for fingerprinting, Identification, and quantification of marker compounds, adulterants, and falsification.
- Pharmaceutical applications for identification and impurity test.
- Food and cosmetic industry – Aflatoxins, Lipids, Carbohydrates, Food Colors, pigments, other additives
- Environmental analysis – Pesticides, PAH’s
- Forensics/toxicology
High-performance thin-layer Chromatography (HPTLC) is a sophisticated analytical technique used for the separation, identification, and quantification of compounds in a mixture. HPTLC testing services are often provided by specialized laboratories. Here's a general overview of the process involved in HPTLC testing services:
Sample Submission:
Clients submit their samples to the testing laboratory along with relevant information such as the type of analysis required, the purpose of the analysis, and any specific parameters or compounds of interest.
Sample Preparation:
The laboratory prepares the samples for analysis. This may involve extraction, purification, or other sample preparation techniques depending on the nature of the sample and the compounds being analyzed.
Standard Preparation:
If quantitative analysis is required, standards of known concentrations are prepared. These standards are used to create a calibration curve for the quantification of the target compounds.
Chromatography Plate Preparation:
The stationary phase (thin layer) is coated on a glass or aluminum plate. The type of stationary phase used depends on the nature of the compounds being analyzed. Silica gel is a common stationary phase.
Application of Sample:
The prepared sample is applied to the chromatography plate using a suitable technique, such as spotting or spraying. The sample should be applied as a narrow band to ensure proper separation.
Chromatographic Development:
The chromatography plate is placed in a developing chamber with a solvent system. As the solvent travels up the plate, it carries the sample components along, causing them to separate based on their affinity for the stationary phase.
Drying:
After the development is complete, the plate is dried to remove any residual solvent.
Detection:
Detection can be done by various means, such as UV absorption, fluorescence, or derivatization. The separated compounds appear as spots on the plate, and their characteristics are used for identification.
Documentation and Analysis:
The chromatogram obtained is documented, and the spots corresponding to different compounds are analyzed. If quantitative analysis is required, the intensity of the spots is compared to the calibration curve generated from the standards.
Reporting:
The results are compiled into a report and sent to the client. The report includes details of the analysis, including the identified compounds, their concentrations (if applicable), and any other relevant information.
Quality Control:
Quality control measures are implemented throughout the process to ensure the accuracy and reliability of the results.
It's important to note that the specifics of the process may vary depending on the equipment, reagents, and methods used by the testing laboratory. HPTLC is a versatile technique, and its applications range from pharmaceutical analysis to food and environmental testing.
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