Synthetic Food Dyes Analysis by HPLC

🔬 High-Performance Liquid Chromatography (HPLC) is a powerful technique used to separate and quantify synthetic dyes in food samples.

🧪 Brief Procedure:

💧 Sample Prep: Food sample is homogenized and extracted using water or a suitable solvent.

🧂 Filtration: The extract is filtered to remove particulates.

💉 Injection: A small volume is injected into the HPLC system.

⚗️ Separation: The dyes are separated on a C18 column using a gradient mobile phase.

📊 Detection: Dyes are detected using UV-Vis at specific wavelengths.

✅ Used in quality control to ensure dye levels comply with food safety standards.

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