Every living being uses Amino acids to make proteins. They help grow and repair body tissue and boost your immune system. It is crucial to balance Essential Amino acid levels throughout the day. There are six tests for determining the functional groups in amino acids. They are: (1) Ninhydrin Test The free amino group and Carboxylic acid group react with ninhydrin to produce a colored residue. (2) Biuret Test Also known as Piotrowski’s Test, the dark violet-colored residue signifies the presence of protein in the solution. (3) Xanthoproteic Test This test identifies the presence of an activated benzene ring in the amino acid. Nitric acid is added to 1 ml of amino acid solution, followed by boiling to detect the Essential Amino acid levels. (4) Millon’s Test This test is suitable for amino acids containing a phenol group. Millon’s reagent is used in the test to identify protein traces in the sample. (5) Hopkins-Cole Test This test is done particul...