Gram Staining | Microbiology 🔬 | fundamental microbiology technique

Discover how bacteria reveal their identity through color! 🎨✨

Gram staining is a fundamental microbiology technique used to classify bacteria as Gram-positive (🟣 purple) or Gram-negative (🔴 pink) based on their cell wall structure.

🔍 Procedure:

1️⃣ Prepare and heat-fix the smear

2️⃣ Add crystal violet (1 min) – rinse

3️⃣ Add iodine (1 min) – rinse

4️⃣ Decolorize with alcohol (few sec) – rinse

5️⃣ Counterstain with safranin (1 min) – rinse & dry

6️⃣ View under microscope 🧪🔬

✅ Gram +: Thick peptidoglycan, retains crystal violet → Purple

✅ Gram -: Thin wall, loses violet, takes safranin → Pink

#Microbiology #GramStaining #ScienceLab #Microscopy #Bacteria

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