The pour plate method is a microbiological technique used to isolate and count viable microorganisms in a sample.
🔬 Procedure:
1️⃣ Prepare sterile Petri dishes.
2️⃣ Pour melted, cooled agar (45°C) into each dish.
3️⃣ Add 1 mL of diluted microbial sample.
4️⃣ Gently swirl to mix evenly.
5️⃣ Let it solidify, then incubate at 37°C for 24-48 hours.
6️⃣ Count colonies formed within and on the surface of the agar.
✅ Ideal for enumerating bacteria in food, water, and clinical samples.
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