Indole Test in Microbiology... #science #chemistry

Indole Test Procedure:

Inoculation:

Using a sterile inoculating loop, transfer a loopful of the bacterial culture into a tube of peptone water or tryptophan broth.

Incubation:

Incubate the tube at 35–37°C for 24 to 48 hours.

Addition of Reagent:

After incubation, gently add 5 drops of Kovac’s reagent down the inner wall of the test tube without shaking.

Observation:

Allow the tube to stand undisturbed for a few minutes.

A red or pink ring at the top indicates a positive indole test.

No color change indicates a negative result.

🎯 Why is the Indole Test Important?

Helps identify indole-producing bacteria

Used in medical and clinical diagnostics

Commonly applied in water and food safety testing

#IndoleTest #Microbiology #ScienceExperiment #ChemistryLab

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