Membrane Filter Method.... #sciencereels #analyticalchemistry

The Membrane Filter Method is a widely used microbiological technique for detecting and counting bacteria in water and liquid samples.

🔬 Procedure :

Take a measured volume of the sample (usually 100 mL).

Pass it through a sterile membrane filter (0.45 µm pore size) using a vacuum pump.

Place the filter carefully onto a selective agar plate.

Incubate the plate at the required temperature (typically 35–37°C) for 24–48 hours.

After incubation, count the colonies that have grown on the filter surface.

Calculate the result as CFU per 100 mL of sample.

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