OXIDASE TEST #sciencereels

OXIDASE TEST.....

In this short video, we demonstrate the oxidase test a well-known oxidase-positive bacterium. This test is used to detect the presence of the enzyme cytochrome c oxidase, which is involved in the electron transport chain.

🧪 Procedure:

Take a clean, sterile oxidase disc or filter paper.

Using a sterile loop or wooden stick, pick a small colony of Pseudomonas from a fresh agar plate.

Rub the colony gently on the disc or moistened filter paper.

Observe the color change within 10–30 seconds.

✅ Positive Result: A purple or dark blue color appears — indicates Pseudomonas is oxidase positive.

🧫 Test Organism: Pseudomonas spp.

🔬 Application: Biochemical Identification of Bacteria

📍 Category: Microbiology | Biochemistry | Chemistry Practical

#Oxidase

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