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Showing posts from July, 2025

Types of Laboratory Tests For Edible Oils

Types of Laboratory Tests for Edible Oils Testing edible oils ensures they are safe, pure, and of high quality. These tests are crucial for FSSAI compliance , export standards, and consumer trust. Physicochemical Tests These tests determine the basic physical and chemical properties of oils. Test Purpose Moisture & Volatile Matter Indicates shelf life and spoilage risk Refractive Index Identifies purity and type of oil Specific Gravity Differentiates between oil types Color (Lovibond Tintometer) Assesses oil clarity and grade Viscosity Measures flow properties   2. Chemical Quality Tests These determine freshness, oxidation level, and chemical degradation. Test Purpose Acid Value Indicates free fatty acids; higher value means rancidity Peroxide Value Measures primary oxidation; important for shelf life Saponification Value Determines molecular weight and type of oil Iodine Value Assesses unsaturation; helps classify the oil Unsaponifiable Matter Detects impurities like...

ICP-MS (Inductively Coupled Plasma Mass Spectrometry)

Your go-to technique for ultra-trace metal analysis! Used in testing water, food, soil , cosmetics & more! ๐ŸŒ๐Ÿ’ง๐ŸŒฟ ⚗️ How It Works: 1️⃣ The sample is converted into an aerosol and introduced into a high-temperature plasma (6000–10,000 K). 2️⃣ In the plasma, the elements are ionized. 3️⃣ These ions are then separated based on their mass-to-charge ratio using a mass spectrometer. 4️⃣ Finally, a detector quantifies each element with extreme precision (as low as parts per trillion!). ✅ Applications: Heavy metals in food ๐Ÿฒ Water quality testing ๐Ÿ’ง Environmental monitoring ๐ŸŒฑ Pharma and cosmetics ๐Ÿงด ๐Ÿ“Œ High sensitivity | Fast results | Multi-element detection #ICPMS #MassSpectrometry #LabTesting #ElementalAnalysis #FoodSafety #WaterTesting #AnalyticalChemistry #ScienceReels #NABL #TraceAnalys

Gram Staining | Microbiology ๐Ÿ”ฌ | fundamental microbiology technique

Discover how bacteria reveal their identity through color! ๐ŸŽจ✨ Gram staining is a fundamental microbiology technique used to classify bacteria as Gram-positive (๐ŸŸฃ purple) or Gram-negative (๐Ÿ”ด pink) based on their cell wall structure. ๐Ÿ” Procedure: 1️⃣ Prepare and heat-fix the smear 2️⃣ Add crystal violet (1 min) – rinse 3️⃣ Add iodine (1 min) – rinse 4️⃣ Decolorize with alcohol (few sec) – rinse 5️⃣ Counterstain with safranin (1 min) – rinse & dry 6️⃣ View under microscope ๐Ÿงช๐Ÿ”ฌ ✅ Gram +: Thick peptidoglycan, retains crystal violet → Purple ✅ Gram -: Thin wall, loses violet, takes safranin → Pink #Microbiology #GramStaining #ScienceLab #Microscopy #Bacteria   

Synthetic Food Dyes Analysis by HPLC

๐Ÿ”ฌ High-Performance Liquid Chromatography (HPLC) is a powerful technique used to separate and quantify synthetic dyes in food samples. ๐Ÿงช Brief Procedure: ๐Ÿ’ง Sample Prep: Food sample is homogenized and extracted using water or a suitable solvent. ๐Ÿง‚ Filtration: The extract is filtered to remove particulates. ๐Ÿ’‰ Injection: A small volume is injected into the HPLC system. ⚗️ Separation: The dyes are separated on a C18 column using a gradient mobile phase. ๐Ÿ“Š Detection: Dyes are detected using UV-Vis at specific wavelengths. ✅ Used in quality control to ensure dye levels comply with food safety standards.

Methacrylate Content Analysis in Polymers Using HPLC #sciencereels

Methacrylate Content Analysis in Polymers Using HPLC High-Performance Liquid Chromatography (HPLC) is a reliable technique used to quantify methacrylate monomers in polymer samples, ensuring product quality, safety, and consistency. Procedure : Sample Preparation: Dissolve the polymer in a suitable solvent (e.g., acetonitrile or methanol). Filtration: Filter the solution to remove any undissolved particles. Injection: Inject a measured volume into the HPLC system. Separation: Methacrylate components are separated using a C18 column with an appropriate mobile phase (e.g., water:acetonitrile). Detection: Detect and quantify using UV detection at around 210–220 nm. Commonly used in material quality control, biomedical polymer validation, and dental resin analysis. #HPLC #Methacrylate #PolymerAnalysis #tamilnadutesthouse #MaterialScience #Chromatography #AnalyticalChemistry #LabTechniques #ScientificResearch

Pour Plate Method part 1 | Microbiology Technique

The pour plate method is a microbiological technique used to isolate and count viable microorganisms in a sample. ๐Ÿ”ฌ Procedure: 1️⃣ Prepare sterile Petri dishes. 2️⃣ Pour melted, cooled agar (45°C) into each dish. 3️⃣ Add 1 mL of diluted microbial sample. 4️⃣ Gently swirl to mix evenly. 5️⃣ Let it solidify, then incubate at 37°C for 24-48 hours. 6️⃣ Count colonies formed within and on the surface of the agar. ✅ Ideal for enumerating bacteria in food, water, and clinical samples. #Microbiology #PourPlate #LabTechniques #BacteriaCulture #ScienceReels #AgarPlate #MicrobialTesting #trending  #viralreels See less

Preservatives Profiling in Food Products | Shimadzu HPLC

Preservatives Profiling in Food Products | Shimadzu HPLC Ensuring food safety with precision! Using High Performance Liquid Chromatography (HPLC) , we identify and quantify common food preservatives like benzoic acid, sorbic acid, and parabens. Shimadzu HPLC offers accurate, reliable, and reproducible results for regulatory compliance and quality assurance. ๐Ÿ”ฌ Procedure Overview: 1️⃣ Sample preparation & filtration 2️⃣ Injection into Shimadzu HPLC system 3️⃣ Separation using C18 column 4️⃣ Detection via UV at specific wavelengths 5️⃣ Comparison with standard preservatives for identification & quantification

HPTLC Identification: Total Organic Carbon (TOC) Analysis

  Total Organic Carbon (TOC) Analysis A key parameter to measure organic contamination in water ๐Ÿ’ง ๐Ÿ” Procedure: Sample is acidified to remove inorganic carbon (like carbonates). It’s then oxidized (chemically or by combustion). The resulting CO₂ is measured to determine the TOC level. ๐Ÿ“Š Why It Matters? TOC indicates water purity & helps monitor environmental and industrial processes ๐ŸŒฑ๐ŸŒ ✅ Common in: Pharma | Environmental Testing | Drinking Water Analysis Read More : https://tamilnadutesthouse.com/hptlc-testing-lab/

๐Ÿ”ฌ FAME Analysis by Gas Chromatography (GC) Part 2

๐Ÿ”ฌ FAME Analysis by Gas Chromatography (GC) Part 2 .... Unlocking the fatty acid profile in biodiesel, food, and biological samples! ๐Ÿ’ก What is it? FAME analysis identifies and quantifies fatty acids after converting them to their methyl esters. It's widely used in biodiesel quality control and nutritional research. ๐Ÿงช Procedure Highlights: 1️⃣ Lipids are extracted from the sample. 2️⃣ Fatty acids are converted to methyl esters (FAMEs) via transesterification. 3️⃣ Injected into the GC system. 4️⃣ GC separates and detects each FAME based on retention time. 5️⃣ Results show detailed fatty acid composition. ✅ Accurate | ๐Ÿ” Reproducible | ๐Ÿ“Š Essential for QC & R&D #FAMEAnalysis #GasChromatography #BiodieselTesting #LabWork # Trendingnow #ScienceInAction #Shimadzu #viralreels #AnalyticalChemistry #FattyAcids #GCMS #LaboratoryLife #TrendingScience 

FAME Analysis by Gas Chromatography (GC) part 1

FAME Analysis by Gas Chromatography (GC) part 1 Unlocking the fatty acid profile in biodiesel, food, and biological samples! ๐Ÿ’ก What is it? FAME analysis identifies and quantifies fatty acids after converting them to their methyl esters. It's widely used in biodiesel quality control and nutritional research. ๐Ÿงช Procedure Highlights: 1️⃣ Lipids are extracted from the sample. 2️⃣ Fatty acids are converted to methyl esters (FAMEs) via transesterification. 3️⃣ Injected into the GC system. 4️⃣ GC separates and detects each FAME based on retention time. 5️⃣ Results show detailed fatty acid composition. ✅ Accurate | ๐Ÿ” Reproducible | ๐Ÿ“Š Essential for QC & R&D

Microbiology Testing at Tamilnadu Test House:

 Ensure your products are safe, pure, and compliant with our advanced microbiological analysis services! At Tamilnadu Test House , we test a wide range of samples—food, water, pharmaceuticals, cosmetics, and AYUSH —for microbial contamination including: ✅ Total Viable Count (TVC) ✅ Yeast & Mold ✅ Pathogens like E. coli, Salmonella, Pseudomonas, Staphylococcus aureus ✅ Preservative Efficacy Test ✅ Sterility Testing We follow IS, IP, BIS, and international guidelines with NABL-accredited protocols. ๐Ÿ“ Trust us to deliver accurate, timely, and reliable results.  

Moisture Content Determination in Soil

Ever wondered how much water your soil holds? ๐ŸŒฑ Here's a simple lab method to find out! ๐Ÿงช Procedure: Weigh an empty container (W1). Add soil sample and weigh again (W2). Dry the soil in a hot air oven at 105–110°C for 24 hours. Cool in a desiccator & weigh (W3). Calculate: ๐Ÿ’ก Moisture Content (%) = ((W2 - W3) / (W3 - W1)) × 100 ๐Ÿ“Œ Why It Matters: Soil moisture affects plant growth, construction stability, and irrigation planning.