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Understanding Total Polar Compounds in Oil: A Key Indicator of Oil Quality and Safety

In the food and oil processing industry, maintaining the quality and safety of edible oils is crucial. One of the most significant indicators of oil degradation during use, especially during frying, is the formation of Total Polar Compounds (TPCs) . These compounds play a vital role in determining whether the oil is still suitable for consumption or needs to be discarded. What Are Total Polar Compounds? Total Polar Compounds are a group of substances formed when edible oils are exposed to high temperatures, such as during deep frying. Over time, heat, oxygen, and moisture cause the oil to break down, resulting in the formation of polar substances like oxidized triglycerides, free fatty acids, mono- and diglycerides, and polymerized triglycerides. These compounds increase with repeated oil use and indicate oil degradation, reduced nutritional value, and the potential formation of harmful substances. Why Is TPC Measurement Important? Monitoring TPC levels is essential for both food safe...
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Indole Test in Microbiology... #science #chemistry

Indole Test Procedure: Inoculation: Using a sterile inoculating loop, transfer a loopful of the bacterial culture into a tube of peptone water or tryptophan broth. Incubation: Incubate the tube at 35–37°C for 24 to 48 hours. Addition of Reagent: After incubation, gently add 5 drops of Kovac’s reagent down the inner wall of the test tube without shaking. Observation: Allow the tube to stand undisturbed for a few minutes. A red or pink ring at the top indicates a positive indole test. No color change indicates a negative result. 🎯 Why is the Indole Test Important? Helps identify indole-producing bacteria Used in medical and clinical diagnostics Commonly applied in water and food safety testing #IndoleTest #Microbiology #ScienceExperiment #ChemistryLab 
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Membrane Filter Method.... #sciencereels #analyticalchemistry

The Membrane Filter Method is a widely used microbiological technique for detecting and counting bacteria in water and liquid samples. 🔬 Procedure :  Take a measured volume of the sample (usually 100 mL). Pass it through a sterile membrane filter (0.45 µm pore size) using a vacuum pump. Place the filter carefully onto a selective agar plate. Incubate the plate at the required temperature (typically 35–37°C) for 24–48 hours. After incubation, count the colonies that have grown on the filter surface. Calculate the result as CFU per 100 mL of sample.
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OXIDASE TEST #sciencereels

OXIDASE TEST..... . . . In this short video, we demonstrate the oxidase test  a well-known oxidase-positive bacterium. This test is used to detect the presence of the enzyme cytochrome c oxidase, which is involved in the electron transport chain. 🧪 Procedure: Take a clean, sterile oxidase disc or filter paper. Using a sterile loop or wooden stick, pick a small colony of Pseudomonas from a fresh agar plate. Rub the colony gently on the disc or moistened filter paper. Observe the color change within 10–30 seconds. ✅ Positive Result: A purple or dark blue color appears — indicates Pseudomonas is oxidase positive. 🧫 Test Organism: Pseudomonas spp. 🔬 Application: Biochemical Identification of Bacteria 📍 Category: Microbiology | Biochemistry | Chemistry Practical #Oxidase
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Types of Laboratory Tests For Edible Oils

Types of Laboratory Tests for Edible Oils Testing edible oils ensures they are safe, pure, and of high quality. These tests are crucial for FSSAI compliance , export standards, and consumer trust. Physicochemical Tests These tests determine the basic physical and chemical properties of oils. Test Purpose Moisture & Volatile Matter Indicates shelf life and spoilage risk Refractive Index Identifies purity and type of oil Specific Gravity Differentiates between oil types Color (Lovibond Tintometer) Assesses oil clarity and grade Viscosity Measures flow properties   2. Chemical Quality Tests These determine freshness, oxidation level, and chemical degradation. Test Purpose Acid Value Indicates free fatty acids; higher value means rancidity Peroxide Value Measures primary oxidation; important for shelf life Saponification Value Determines molecular weight and type of oil Iodine Value Assesses unsaturation; helps classify the oil Unsaponifiable Matter Detects impurities like...
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ICP-MS (Inductively Coupled Plasma Mass Spectrometry)

Your go-to technique for ultra-trace metal analysis! Used in testing water, food, soil , cosmetics & more! 🌍💧🌿 ⚗️ How It Works: 1️⃣ The sample is converted into an aerosol and introduced into a high-temperature plasma (6000–10,000 K). 2️⃣ In the plasma, the elements are ionized. 3️⃣ These ions are then separated based on their mass-to-charge ratio using a mass spectrometer. 4️⃣ Finally, a detector quantifies each element with extreme precision (as low as parts per trillion!). ✅ Applications: Heavy metals in food 🍲 Water quality testing 💧 Environmental monitoring 🌱 Pharma and cosmetics 🧴 📌 High sensitivity | Fast results | Multi-element detection #ICPMS #MassSpectrometry #LabTesting #ElementalAnalysis #FoodSafety #WaterTesting #AnalyticalChemistry #ScienceReels #NABL #TraceAnalys
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Gram Staining | Microbiology 🔬 | fundamental microbiology technique

Discover how bacteria reveal their identity through color! 🎨✨ Gram staining is a fundamental microbiology technique used to classify bacteria as Gram-positive (🟣 purple) or Gram-negative (🔴 pink) based on their cell wall structure. 🔍 Procedure: 1️⃣ Prepare and heat-fix the smear 2️⃣ Add crystal violet (1 min) – rinse 3️⃣ Add iodine (1 min) – rinse 4️⃣ Decolorize with alcohol (few sec) – rinse 5️⃣ Counterstain with safranin (1 min) – rinse & dry 6️⃣ View under microscope 🧪🔬 ✅ Gram +: Thick peptidoglycan, retains crystal violet → Purple ✅ Gram -: Thin wall, loses violet, takes safranin → Pink #Microbiology #GramStaining #ScienceLab #Microscopy #Bacteria   
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